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Implications of ADAM Modulation of Integrin-mediated Cell Migration
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Analysis of movies showed that mock transfected cells displayed broad lamellipodia at the leading edge and the cells moved persistently in the direction of the protrusion (Movie 2A). As seen in Figure 7, mock-transfected cells migrated persistently at a rate of ∼10 μm/h. Twenty-four hours after transfection, cells were labeled with the membrane-impermeable EZ-Link NHS-LC-biotin (Pierce Chemical), and avidin precipitation and immunoblotting were conducted as described in Materials and Methods. CHO-α4/GFP cells were transfected with 4 μg of DNA (per 6-cm dish) encoding the indicated 6Xmyc-tagged constructs.